THE 38TH ANNUAL MEETING OF THE JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY
Cytochrome c Oxidase Activity of Large Mitochondria Appeared in Osteoclasts during the Early Stage of Parathyroid Hormone Treatment
Koji Noda, Taeko Yoshii, Yoshiki Nakamura and Yosuke Kuwahara
Department of Orthodontics, School of Dental Medicine, Tsurumi University, 2-1-3, Tsurumi, Tsurumi-ku, Yokohama, Kanagawa 230-8501
Received for publication October 20, 1997 and in revised form February 5, 1998
Large mitochondria that appeared in osteoclasts during the early stage of parathyroid hormone (PTH) treatment were examined histochemically and morphologically in order to elucidate how their appearance is related to ruffled border (RB) form or cytochrome c oxidase activity. Three types of osteoclasts including large mitochondria, that were characterized by closely packed cristae, appeared 1-2 hr after PTH treatment. The first type (PTH-1) showed a poorlydeveloped RB, and many large mitochondria concentrated on the basal membrane side. The second type (PTH-2) showed a welldeveloped RB, but the terminal infoldings did not expand. Many large mitochondria were scattered freely in PTH-2. The third type (PTH3) showed a well-developed RB, and many expanded terminal infoldings and vacuoles could be seen in the RB. Large mitochondria in PTH-3 were few in number. Cytochrome c oxidase activities both in the control and in PTH3 osteoclasts were significantly higher than that in the PTH-1 osteoclasts. Over 90% of large mitochondria showed high enzymatic activity. Only 0.7% of control osteoclasts had a cross-sectional mitochondrial area of over 0.867 $\mu$m2 (large mitochondria), whereas the area of over 0.867 $\mu$m2 was 4.7% of PTH-1, 9% of PTH-2, and 2.3% of PTH-3 respectively. The present results
suggest that large mitochondria demonstrate high ATP production, and that a close relationship exists between the location and number of large mitochondria and RB form during the early period when osteoclastic bone resorption is promoted by PTH.
Key words: Cytochrome c oxidase, Osteoclast, Large mitochondria, Parathyroid hormone, ATP
Localization of 2,2,7-trimethylguanosine, SC-35 and TIAR in Ameloblast Nuclei of the Rat Incisor, and Their Disappearance during Apoptosis
Sumio Nishikawa and Fumie Sasaki
Department of Biology, Tsurumi University School of Dental Medicine, Yokohama 230-8501
Received for publication December 5, 1997 and in revised form January 26, 1998
Spliceosomal components, U class snRNA and SC-35, were investigated using anti-2,2,7-trimethylguanosine (m3G) antibody for snRNA and anti-SC-35 antibody by
immunofluorescence and immunoelectron microscopy in normal and apoptotic ameloblasts. Nuclear substructures, interchromatin granule clusters (IGCs) and perichromatin fibrils (PFs), were positive to anti-m3G antibody and anti-SC-35 antibody. Most of the apoptotic nuclei were not labeled by either antibody, although some were labeled by immunofluorescence microscopy. In earlier apoptotic nuclei, anti-m3G localized in a scattered pattern in the interchromatin space in immunoelectron microscopy. TIAR, a member of the RNA binding proteins with an RNA recognition motif and an effector of apoptosis, localized in the normal ameloblast nucleus, but was mostly lacking in the apoptotic nuclei, excepting occasional diffuse labeling in immunofluorescence microscopy. These results suggest that in addition to nuclear chromatin condensation, rearrangement of interchromatin space including IGCs and PFs occurs during apoptosis, further implying that the modulation of gene transcription and splicing may be accompanied by nuclear changes during the process.
Histochemical Analysis Using Lectin in the Mouse Uterine Tissues with
Experimentally-Induced Adenomyosis
Haruko Sasabe, Manabu Matsuda and Takao Mori
Department of Biological Sciences, Graduate School of Science, University of Tokyo, Bunkyo-ku, Tokyo 113
Received for publication January 5, 1998 and in revised form February 9, 1998
Transplantation of a single anterior pituitary into the uterine lumen is known to induce the development of adenomyosis in mice associated with hyperprolactinemia. The uterine tissues of experimentally-induced adenomyosis were investigated histochemi- cally with 21 kinds of biotinylated lectins and compared to control tissues of both intact mice and mice one day after parturition. Glandular and luminal epithelial cells and lymphocytes showed different lectin staining patterns between adenomyotic uteri and intact ones, whereas endometrial stromal cells and myometrial cells showed no differ- ences. Even uteri at a very early stage of the pathological disorder already had changes in their lectin binding pattern. Some lectin groups had a different staining pattern between adenomyotic uteri and intact ones. Among them, some showed a similar pattern between adenomyotic uteri and uteri after delivery, and the others showed a different pattern between them. The former groups were closely related to an elevated level of circulating prolactin, and the latter to a pathological change in adenomyosis. This is the first evidence which shows that experimentally-induced adenomyosis involves substantial changes even at a very early stage of the pathogenesis.
1 First Department of Oral and Maxillofacial Surgery and 2 Department of Oral Pathology, Faculty of Dentistry, Kyushu University, 3-1-1, Maidashi, Higashi-ku, Fukuoka, 812-8582
Received for publication January 5, 1998 and in revised form February 23, 1998
This study was performed to investigate the cellular interactions between three different suture materials including Poliglecaprone 25 (Monocryl, MN), Polyglycolic acid (Dexon, DX) and Blacksilk (BS), and living tissue regarding the absorption process and bio- compatibility of the thread, and to determine the localization of macrophages using ED1 monoclonal antibody. These threads were applied in gluteal muscle closures of thirty-six male Wistar strain rats. Both histological and immunohistochemical observations were performed at the following intervals: 1, 3, 6, 9, 12, and 24 weeks, postoperatively. The absorption of DX occurred in the early experimental phase by the penetration of various amount of ED1-positive macrophages. The absorption of MN was delayed compared with that of the DX, while many ED1-positive macrophages penetrated inside the MN in the mid-experimental phases. Both MN and DX were substantially absorbed at 24 weeks postoperatively with minimal scar tissue. Meanwhile, the BS still remained in the muscular tissue at 24 weeks post- operatively, with a remarkable proliferation of fibrous tissue around the thread. This study suggested that the ED1-positive macro- phages played a central role of absorption of MN and DX, and that the MN could possess a superior healing response with a minimum of scar formation.